Judith of Habsburg (German: Guta; 13 March 1271 – 21 May 1297) was queen of Bohemia and Poland from 1285 until her death as the wife of the Přemyslid king Wenceslaus II.

After conflicts arose with Ottokar's widow Kunigunda of Halych, Margrave Otto temporarily held Wenceslaus as a prisoner at Bezděz Castle and in the Ascanian fortress of Spandau in Brandenburg.

As part of a reconciliation process, the formal engagement between Judith and Wenceslaus was renewed in 1279 at Jihlava; nevertheless, the bridal couple did not meet until in January 1285 a wedding ceremony was held by the Přemyslid and Habsburg dynasties in the City of Cheb (Eger).

Though Kunigunda died later in that year and Wenceslaus II had sworn an oath of fealty to Rudolf in order to receive his Bohemian heritage, his coronation as king of Bohemia had to be postponed as Judith was not present.

She supported her husband's claim on the Kingdom of Poland, where he ruled over the Seniorate Province at Kraków since 1291 and was able to succeed King Przemysł II in 1296.