Julius Nicolaysen (31 July 1831 – 25 December 1909) was a Norwegian professor of medicine.
He was born in Bergen as a son of merchant and banker Lyder Wentzel Nicolaysen (1794–1876) and Cathrine Margrethe Wilhelmine Bernhoft (1808–1871).
[1] In April 1859 in Borre he married his first cousin Bolette Marie Berentine Nicolaysen (1835–1922).
He was hired at Rikshospitalet in 1863; from 1867 to 1870 he was a research fellow at the university (including thirteen months in the United States).
He also contributed significantly to the introduction of antiseptic, later aseptic methods in surgery.