Junction City is a village in Madison County, Missouri, United States.

State Highway OO leads north from Junction City 18 miles (29 km) to Farmington.

There were 179 housing units at an average density of 497.2 per square mile (192.0/km2).

The racial makeup of the village was 96.94% White, 0.61% Black or African American, 0.31% Native Hawaiian or Pacific Islander, and 2.14% from two or more races.

44.7% of all households were made up of individuals, and 33.3% had someone living alone who was 65 years of age or older.

48.1% of all households were made up of individuals, and 27.2% had someone living alone who was 65 years of age or older.