Other voice actors of the film are Hailee Steinfeld, Brian Tyree Henry, Lauren Vélez, Jake Johnson, Jason Schwartzman, Issa Rae, Karan Soni, Shea Whigham, Greta Lee, Daniel Kaluuya, Mahershala Ali, and Oscar Isaac.

Miles goes on an adventure with Gwen Stacy / Spider-Woman (Steinfeld) across the multiverse, where he meets a team of Spider-People led by Miguel O'Hara / Spider-Man 2099 (Isaac) known as the Spider-Society, but comes into conflict with them over handling a new threat in the form of the Spot (Schwartzman).

Gwen encounters a version of the Vulture from an Italian Renaissance–themed alternate universe, and Miguel O'Hara and Jess Drew arrive through dimensional portals to help neutralize him.

In Brooklyn on Earth-1610, 16 months after Kingpin's defeat and the destruction of the Alchemax collider,[a] Miles Morales is having trouble with his parents due to his duties as Spider-Man interfering with his personal life, and is grounded when he is late to a party celebrating his father Jeff's promotion to police captain.

The Spot absorbs the power of the collider, and he and Miles share a vision of his future attacks, which include the deaths of Jeff and Police Inspector Singh, the father of Pavitr's girlfriend Gayatri.

Gwen, after promising to Rio and Jeff that she will find Miles, teams up with Peter B., Mayday, Pavitr, Hobie, Margo, Spider-Man Noir, Peni Parker, and Spider-Ham.

Additional voice cast includes: Shea Whigham as George Stacy, Gwen's father and a police captain;[27] Jorma Taccone as Adriano Tumino / Vulture, a supervillain from an Italian Renaissance-inspired universe,[18][27] as well as Peter Parker / Spider-Man from the 1967 animated series, which he reprises from the post-credits of Into the Spider-Verse;[28][29] Greta Lee as Lyla, Miguel and the Spider Society's AI assistant;[30] Mahershala Ali as Aaron Davis, the Earth-42 version of Miles' deceased uncle (Ali previously voiced the Earth-1610 version of Aaron in the first film);[31] and Jharrel Jerome as Miles G. Morales / Prowler of Earth-42.

[37] Archival audio of Alfred Molina's Otto Octavius / Doctor Octopus from Spider-Man: No Way Home (2021) is used for a hologram version of the character with a comic book-accurate design.

[52] By the end of November 2018, ahead of the release of Spider-Man: Into the Spider-Verse (2018) the following month, Sony Pictures Animation had begun developing a sequel[53] due to the "incredible buzz" surrounding the project.

[62] Characters that were considered to appear in Across the Spider-Verse but were ultimately rejected include versions of Green Goblin, Doctor Octopus, Kraven the Hunter, Mysterio, Electro, Shocker, Rhino, Hobgoblin, Chameleon, Puma, Leap-Frog, Alistair Smythe, Grizzly, Boomerang, Screwball, Big Wheel, Beetle, and Diamondhead.

[citation needed] One of the two alternate Earths seen in the trailer was Earth-50101, which the crew nicknamed "Mumbattan" after Mumbai and Manhattan, due to that world being based on the one from Gotham Entertainment Group's Spider-Man: India comic book series.

The city was created as a highly controlled environment reflecting Miguel's personality, and has an authoritarian, brutalist and minimalist style of design meant to represent a "clean, strong, unified, front-facing ideology".

[84][88] Spider-Punk's animation style took two to three years to develop and is inspired by collage imagery of 1970s punk-rock album covers, posters, and zines emulating the look of Xerox prints, colored papers and images appropriated from magazines and newsprints, and has a general grayed-out quality due to copy machines not having toner in them.

[98] Additionally, Donald Glover reprises his role as an alternate version of Aaron Davis / Prowler from the MCU film Spider-Man: Homecoming (2017) in a live-action cameo, depicted as a prisoner of the Spider-Society displaced from his home reality.

Sony denied the allegations concerning Across the Spider-Verse's production troubles, while declining to comment on the possibility of the sequel being delayed, and producer Amy Pascal called the process of heavy revision normal.

[better source needed] Though external to the film's score and soundtrack, Daniel Kaluuya also incorporated musical elements into his vocal performance as Spider-Punk / Hobie Brown.

[106] Following the announcement of the new title and release date in April 2022, Lord and Miller showed 15 minutes of the film (with unfinished animation) at Sony's CinemaCon panel.

[111] Meanwhile, Abid Rahman from The Hollywood Reporter compared Miles' introduction to the Spider-People in the trailer to the "vibe" of Citadel of Ricks and highlighted the reference to the Spider-Man pointing meme.

[116] Spider-Man: Across the Spider-Verse premiered on May 30, 2023, at the Grand Rex in Paris, France,[117] and at the Regency Village Theater in Los Angeles,[118] and was theatrically released in the United States on June 2, in premium large formats and IMAX.

[149] The film would make $19.3 million on its fourth weekend, topping the box office once again amidst competition from No Hard Feelings, a new release, and The Flash and Elemental, both holdovers.

[146] Brian Tallerico of RogerEbert.com gave the film 4 out of 4 stars, writing, "Like the work of a young artist who refuses to be restrained by the borders of the frame, Across the Spider-Verse is loaded with incredible imagery and fascinating ideas.

"[157] Ann Hornaday of The Washington Post gave the film 3/4 stars, calling it "an extravagant, very cool love letter to graphic design, executed with superb draftsmanship and giddy, infectious joy.

"[158] Richard Roeper of the Chicago Sun-Times also gave it 3 out of 4 stars, writing, "It's a bit too much at times, but it's also pretty great, and it'll be a huge surprise if the third chapter in the trilogy isn't equally entertaining.

"[159] Kevin Maher of The Times gave the film 4 out of 5 stars, writing, "It rarely draws breath between set pieces, and creates distinct and spectacular environments for each separate spider-verse.

"[160] David Fear of Rolling Stone said that the film "completely recaptures the panel-to-panel thrill of discovering your respective era's teen-angsty Marvel icon.

"[162] The Atlantic's David Sims wrote that the film "avoids seeming like a physics lesson because its visuals are consistently thrilling; the most static bits of dialogue still pop because of how daring the animation is.

[164] Jake Wilson of The Age gave the film 3 out of 5 stars, writing, "Typically for Lord and Miller, the upshot is both adventurous and conformist, an outwardly bold reimagining of the material that never stops congratulating us for our brand loyalty, and which plays it safer in plot terms than the early sequences might lead you to hope.

[53] Pascal said the film would focus on the characters Gwen Stacy / Spider-Gwen, Cindy Moon / Silk, and Jess Drew / Spider-Woman, and said Across the Spider-Verse would serve as a "launching pad" for it.

[191] On May 29, 2023, it was announced that a manga spin-off titled Spider-Man: Octopus Girl written by Hideyuki Furuhashi and illustrated by Betten Court would begin serialization on June 20 on Shueisha's Shōnen Jump+ website and app.

[192][193] In February 2024, Viz Media announced that they licensed the series for English publication in the fourth quarter of 2024 under the title Spider-Man: Octo-Girl (Japanese: スパイダーマン：オクトパスガール, Hepburn: Supaidāman: Okutopasu Gāru).