Metro operates fixed-route bus service on 21 routes, serving the city of Kalamazoo, its immediate suburbs, and Western Michigan University.

Metro's rural services are funded by the Kalamazoo County Transportation Authority, a separate but closely related agency.

Metro's fixed-route system serves the city of Kalamazoo and its immediate suburbs, including Portage and Comstock Township.

Additional transfer points include The Crossroads in Portage, the Western Michigan University main campus, and Maple Hill Pavilion in Oshtemo Township.

[5] The first public motor bus service in Kalamazoo County began in 1905, using an Oldsmobile Ten Passenger Wagonette.

The bus service connected Nazareth Academy with the East Main Street streetcar line's terminus at the Kalamazoo city limits, a distance of approximately one mile (1.6 km).

[9] The newly formed service was branded as "Metro Transit" in October 1967, coinciding with the arrival of a fleet of new GM New Look buses, funded in part by a federal grant.

[9] Metro Transit expanded its services in the 1970s, extending bus lines outside Kalamazoo city limits.

[9] Metro Transit installed automatic passenger counters on its buses in 1980 as part of a demonstration project, funded by the state of Michigan.

[12] A state law passed in 2010 allowed Kalamazoo County to have two transportation authorities, each levying a different property tax rate.

At the same time, the agency began an effort to study its bus stop locations, with the goal of improving passenger experience and service reliability.

Additional transfer points are located at the Western Michigan University main campus, The Crossroads mall in Portage, and Maple Hill Pavilion in Oshtemo Township.

[4] Kalamazoo Transportation Center Crossroads Mall Portage Industrial Park Maple Hill Pavilion Kalamazoo Transportation Center S. 12th St & W. Milham Av Eastwood Plaza Walmart, Gull Rd Maple Hill Pavilion Portage Industrial Park Walmart, Gull Rd Meijer, Gull Rd Kalamazoo Valley Community College Texas Township Campus Meijer, S. Westnedge Ave Township Hall 58 West Apartments Crossroads Mall S. 12th St & W. Milham Av Mulberry Point Apartments A Metro bus was involved in a serious collision on May 24, 2017.