She received her musical training at Dakshinee,[5] a prominent Rabindra Sangeet academy in Kolkata, and obtained a degree in English literature from Jadavpur University,[6] where she also won a Gates Cambridge Scholarship.

[7] She has since performed extensively in India and abroad,[8] including at the North American Bengali Conferences on three occasions – in 2012 (Las Vegas),[9] 2013 (Toronto),[10] and 2016 (New York City).

[citation needed] In 2015, Mukherji presented a solo concert for the President of India, to celebrate the 154th Birth Anniversary of Tagore, at the Rashtrapati Bhavan, New Delhi.

[24]In September 2007, The Telegraph praised her performance at another Dakshinee concert, writing "Yet a musical and humane understanding of the whole life and shape of a song came unmistakably through in the controlled devastation of Kamalini Mukherjee’s Tori amar hothat dubey jai (তরী আমার হঠাৎ ডুবে যায়)".

The Telegraph wrote: [T]he most remarkable element of her gayaki is the precise and powerful scansion — never an inadvertent splitting of a word, or a breather in the wrong place —- that puts her in the tiny club of cerebral Rabindrasangeet singers.

[27] In November 2010, The Telegraph reviewed her experimental concert Nutan juger bhore (নূতন যুগের ভোরে), Thanks to Mukherji’s powerful singing that never allowed a note to waver or an emotional resonance to overstep its suggestion, the music remained as embellishment and never took an iota away from her performance.