Haruka Mizusawa is a meek young man who became infected with Amazon Cells (アマゾン細胞, Amazon Saibō), which are created by the Nozama Pharmacy company (野座間製薬株式会社, Nozama Seiyaku Kabushikigaisha).

Taking place five years after the first season, Season 2 focuses on a new protagonist named Chihiro, a lone boy raised by Amazons who hates them but must suppress his cannibalistic urges and can transform into Amazon Neo, and is soon revealed to be the son of Jin Takayama.

The original protagonist Haruka would later acquire an upgraded form known as Amazon New Omega.

[19] This movie also celebrates the 10th anniversary of Kamen Rider Den-O and features the spaceship Andor Genesis from the Xevious game, which is used by the movie's main antagonists, as well as introduces the movie-exclusive Kamen Rider True Brave, played by Kamen Rider Brave's actor Toshiki Seto from Kamen Rider Ex-Aid, and the villain Shocker Great Leader III, played by the singer Diamond Yukai.

[20] A sequel film titled Kamen Rider Amazons the Movie: The Last Judgement (仮面ライダーアマゾンズ THE MOVIE 最後ノ審判, Kamen Raidā Amazonzu Za Mūbī Saigo no Shinpan), which is set after the end of the second season and concludes the storyline, was released in Japan on May 19, 2018.