It is located south of Lake George and the Kazinga channel in the Albertine Rift eco-region, which is known for its abundance of endemic species.

[2][3][4][5][6] The forest reserve lies within the counties of Bunyaruguru, Igara and Buhweju in the administrative districts of Bushenyi, Ibanda and Kamwenge[2] It was officially gazetted in 1932 and is controlled by Uganda's National Forestry Authority (NFA).

Kasyoha-Kitomi is also home to one Albertine Rift endemic (the Blue-headed Sunbird), Afep Pigeon, Black Bee-eater, Blue-throated Brown Sunbird, Blue-throated Roller, Dusky Long-tailed Cuckoo, White-collared Olive-back, Cinnamon-breasted Bee-eater, Shelley's Greenbul, Equatorial Akalat, and Mountain Illadopsis are among the other species.

[4] Because the majority of the larger trees have been harvested for timber and fire wood, the reserve now contains short shrubs and reeds.

In turn, some communities around the forest reserve have taken advantage of the opportunity to illegally hunt down the animals for meat.