Srebotnik won four singles titles on the WTA Tour and was ranked inside the top 30 for several years.

[2] Srebotnik made her debut on the ITF Women's Circuit in 1995, winning the singles tournaments in Ismailia in 1996, Zadar in 1997 and Šibenik in 1998.

In May 1999, Srebotnik played in her first Grand Slam singles main draw at Roland Garros, losing to Arantxa Sánchez Vicario in the second round.

Srebotnik reached her first Tier-I semifinal in Tokyo at the Pan Pacific Open, which she lost to Sandrine Testud.

She reached the fourth round at Roland Garros, which is her career-best Grand Slam performance.

She won the Bogotá doubles title with Asa Svensson, and reached her second Tier I quarterfinals in Toronto at the Canadian Open.

Srebotnik won her seventh doubles title in Tokyo at the Japan Open (with Shinobu Asagoe).

Her best season to date was highlighted by two singles and four doubles titles as well as a career-best victory over Amélie Mauresmo.

In addition to Auckland and Stockholm, Srebotnik won doubles titles at Budapest and Hasselt (both with Émilie Loit).

She reached the US Open mixed-doubles final (with Nenad Zimonjić, losing to Daniela Hantuchová and Mahesh Bhupathi).

Two weeks later at the Australian Open, with partner Shinobu Asagoe, she made it to the semifinals in doubles, losing to Yan Zi and Zheng Jie.

[4] She won doubles titles in Antwerp[5] (with Dinara Safina) and Amelia Island (with Shinobu Asagoe).

At the French Open, Srebotnik caused an upset when she defeated Serena Williams, whom she had never beaten in four previous attempts, in the third round.

In that year, Srebotnik teamed with Květa Peschke, and won the WTA tournaments of Indian Wells (defeating Nadia Petrova and Sam Stosur in the final) and New Haven (defeating Bethanie Mattek-Sands and Meghann Shaughnessy), and reached the final of the WTA Championships in Doha.

In the ladies' doubles, she and Peschke defeated the second seeds Nuria Llagostera Vives and María José Martínez Sánchez in the semifinals, but lost to Serena and Venus Williams in the final.