[2][3] She previously served as Chief Policy Officer at HackerOne, a vulnerability disclosure company based in San Francisco, California,[4] and currently is the founder and CEO of Luta Security.

[6] She attended Simmons College to study molecular biology and mathematics and simultaneously worked on the Human Genome Project at the MIT Whitehead Institute.

[19] In May 2014, Moussouris was named the Chief Policy Officer at HackerOne, a vulnerability disclosure company based in San Francisco, California.

[4] In this role, Moussouris was responsible for the company's vulnerability disclosure philosophy, and worked to promote and legitimize security research among organizations, legislators and policy makers.

[20] In March 2016, Moussouris was directly involved in creating the Department of Defense's "Hack the Pentagon" pilot program, organized and vetted by HackerOne.

[23] In April 2016,[24] Moussouris founded Luta Security,[25] a consultancy to help organizations and governments work collaboratively with hackers through bug bounty programs.

Moussouris wrote an op-ed in Wired criticizing the move as harmful to the vulnerability disclosure industry due to the overly-broad definition and encouraged security experts to write in to help regulators understand how to make the right changes.

[28] She was invited as a technical expert to directly assist in the US Wassenaar Arrangement negotiations, and helped rewrite the amendment to adopt end-use decontrol exemptions based on the intent of the user.

[33] In 2021, Moussouris donated $1 million to found the Anuncia Donecia Songsong Manglona Lab for Gender and Economic Equity, at Penn State Law, named after her mother.