Bujwidowa was born on 16 October 1867 in Warsaw, Congress Poland, the illegitimate child of Ludwika and Kazimierz Klimontowicz.

She married the bacteriologist and social worker Odo Bujwid in 1886 and became her husband's assistant and laboratory technician.

When Bujwid was appointed as a professor at Jagellonian University in 1893, they moved to Kraków in Austrian Poland and she became an administrator at the Institute for the Production of Sera and Vaccines, headed by her husband.

She also organized the Kraków Reading Room for Women (Polish: Czytelnia dla kobiet) and became its chairwoman.

"[2] Bujwidowa is credited with starting the first junior school for girls and for campaigning to see women entering Jagiellonian University as they did in 1897.