The route is located entirely within Hopkinsville and Christian County.

The highway runs from KY 272 and Camilla Drive on the southwestern side of Hopkinsville to US Route 41 (US 41) north of downtown.

For the vast majority of its route, KY 1007 is known locally as North Drive and runs south-north on the western side of Hopkinsville, serving a mix of residential and industrial areas.

Overall, the highway serves as a sort of local inner bypass on the west side of town.

Near its northern terminus, the route turns onto Sanderson Drive before shortly terminating at US 41.