[2] Her 1990 dissertation was titled Structure and expression of the Petunia mitochondrial S-PCF locus and cytochrome oxidase subunit II genes.

[4] Ostell hired Pruitt to develop a new project to keep track of curated sequences for the human genome.

Pruitt's group curated data and created sequence records for humans, animals, plants, fungi, and bacteria.

[1] In 2017, she became acting chief of the information engineering branch (IEB) at NCBI, where she established a production services operating board to communicate on the status and future directions of critical services, including PubMed, PubMed Central, PubChem, ClinicalTrials.gov, GenBank, BLAST, Pathogen Detection, ClinVar, and dbGaP.

Pruitt leads NCBI's more than 500 highly skilled scientific and technical staff in the design, development, and maintenance of databases, information systems, and software tools, as well as operational management of data submissions, quality checks, and data access.