In January 2006, she played comeback player Martina Hingis, at the WTA Tour tournament in Gold Coast, where she lost in the second round.

In the first round of the Warsaw Open, Koukalová caused a big upset by defeating fourth seed and world No.

She defeated Rossana de los Rios, Tatjana Malek, Sorana Cîrstea, and Li Na en route to the final.

[2] Playing in at the Paris Indoor event, Koukalová lost in the second round to seventh seed Yanina Wickmayer.

She was upset in the first round by Taiwanese qualifier Chan Yung-jan. After her defeat at Roland Garros, Koukalová traveled to Copenhagen to defend her finalist points from the year before.

In the second round, she avenged her Australian Open loss by upsetting 31st seed Lucie Šafářová in three sets.

[5] Seeded seventh at the Hobart International, Koukalová reached the final where she was defeated by qualifier Garbiñe Muguruza.

[8] In Paris at the indoor event, she was defeated in the second round by fourth seed and 2012 champion Angelique Kerber.

[14] Seeded 28th at the Indian Wells Open, Koukalová was defeated in the second round by compatriot Karolína Plíšková.

Koukalová began her clay-court season at the Porsche Tennis Grand Prix where she lost in the first round to eighth seed Sara Errani.

[18] At the Madrid Open, she was defeated in the first round by eighth seed and eventual champion, Maria Sharapova.

[19] Seeded second at the Sparta Prague Open, she lost in the first round to eventual champion Heather Watson.

[20] Seeded third at the Nürnberger Versicherungscup, her final tournament before the French Open, she was defeated in the first round by Polona Hercog.

[23] Seeded eighth at the Rosmalen Open, she advanced to the semifinal round where she lost to eventual champion CoCo Vandeweghe.

[27] In Montreal at the Rogers Cup, Koukalová lost in the second round to 11th seed and 2010 champion Caroline Wozniacki.

[29] Competing at the Connecticut Open, Koukalová lost in the first round to sixth seed Flavia Pennetta.

[35] She played her final tournament of the season at the Kremlin Cup and retired during her first-round match against fifth seed Svetlana Kuznetsova due to illness.

[43] At the Indian Wells Open, she beat two-time champion Daniela Hantuchová in her first-round match.

[46] In Katowice, she lost her quarterfinals match to top seed and home crowd favorite, Agnieszka Radwańska.

[50] Beginning grass-court season at the Rosmalen Open, Koukalová was defeated in the second round by Annika Beck.

[54] Playing in Austria at the Gastein Ladies, Koukalová was defeated in the second round by second seed and eventual champion, Sam Stosur.

[57] In Seoul at the Korea Open, Koukalová retired from her first-round match against Magdaléna Rybáriková due to injury.

Entering the Kremlin Cup as a qualifier, she was defeated in the second round by eventual champion Svetlana Kuznetsova.

[63] Playing at the Indian Wells Open, she was defeated in the first round of qualifying by Verónica Cepede Royg.

[64] Beginning her clay-court season at the Porsche Tennis Grand Prix, Koukalová was beaten in the first round of qualifying by Laura Robson.

Only main-draw results in WTA Tour, Grand Slam tournaments, Fed Cup and Olympic Games are included in win–loss records.