Designed by Donald Kennedy in 1845, the original Knox Church was located in Sandy Hill at the corner of Daly Avenue and Cumberland.

[1] In 1866, a number of members formed a congregation on Bank Street, that is now Dominion-Chalmers United Church, just two blocks west at Cooper and Lisgar.

They now rest in the sanctuary of the present church in a display case with a plaque dedicated to the memory of those who served in the 207th (Ottawa-Carleton) Battalion, CEF during the First World War.

[4] The present Knox Church building, designed in 1931 by John Albert Ewart[5] and Henry Sproatt opened in 1932.

In 2008, Knox again hosted the opening Worship at General Assembly, while the remainder of the activities took place at Carleton University.