The single peaked at number 76 on the US Billboard Hot 100 and was certified Platinum by the Recording Industry Association of America.

[1] David Jeffries of AllMusic labeled it a "pumping party jam" and among the best tracks on the Crime Mob album.

[5] In 2006, during a late-night party at a Howard Johnson hotel ballroom near Fredericksburg, Virginia, a fight on the dance floor began after the DJ played the song, during which 16-year-old Baron "Deuce" Braswell II, who played on the football team at Courtland High School in nearby Spotsylvania, was stabbed to death.

[7] Also in 2007, a fight broke out at an after-party hosted by the Harvard University Society of Black Scientists and Engineers.

[12] In The Rap Year Book, Meaghan Garvey considered "Knuck If You Buck" to be the most important song of 2004: "...its super-essentialized beat and evergreen 'I wish a bitch would' threats never go out of season".

[15] "The Club", a first-season episode of the TV series Atlanta, used the song as background music during a scene at a nightclub.

[16][17] The single "Grandson for President", released on April 29 2020 by rapper King Von, samples the beat to "Knuck If You Buck" In 2024, the song was used in the trailer for the TV series Knuckles.