Labis is a town and a mukim (township) in Segamat District in northern Johor, Malaysia.

A main trunk road that runs north-south Peninsular Malaysia passes through it, as well as the KTM railway line that connects it with the state's capital in the south, Johor Bahru.

In early 20th century, British officers came to the villages to survey for new areas to be developed in Segamat district.

Labis covers an area of 422 km2, making it the second largest mukim in Johor after Bekok.

There is a waterfall known as Taka Melor famous among locals situated 15 km from the town near Pekan Air Panas and Kampung Tenang.