[1] For the sequels and subsequent film and television appearances, Audley was succeeded by American actress Susanne Blakeslee[2][3] who also currently voices Maleficent, Cruella de Vil from 101 Dalmatians, and Queen Grimhilde from Snow White and the Seven Dwarfs.

Cinderella's father, a widower anxious for his daughter to have a mother figure, married Lady Tremaine, whom the narrator describes as "a woman of good family".

After an unspecified amount of time living happily together as a family, Cinderella's father tragically died and Lady Tremaine revealed her true colors.

When Cinderella actually finishes her work and appears ready for the Ball in a suitable dress, Lady Tremaine mildly mentions the beads and sash that Jaq and Gus found after Anastasia and Drizella threw them out.

When Cinderella, with the help of a Fairy Godmother, nevertheless attends the ball, her stepfamily does not recognize her, but Lady Tremaine notes a familiarity about her appearance as she dances with Prince Charming.

News promptly arrives by way of a Royal Proclamation that the Prince will marry the girl whose foot fits the glass slipper accidentally left behind by Cinderella at the ball.

Hoping to pass off one of her daughters as the mysterious girl, Lady Tremaine follows Cinderella up to the attic and locks her in her room, putting the key in her pocket.

But the Duke, who is required by the King's Royal Proclamation not to skip a single maiden in the kingdom on his quest for the mysterious girl the Prince danced with, solemnly rebuffs Lady Tremaine.

In a last-ditch effort to foil Cinderella's dreams, Lady Tremaine trips the footman bearing the glass slipper, causing it to smash.

But Cinderella still manages to come out on top by revealing that she has the other slipper, and that it fits her foot, proving that she is the girl who danced with Prince Charming, much to her stepmother's appalled horror.

Beyond a single brief close-up of her appalled face, the movie does not dwell on her defeat, how she and her daughters further reacted or what happened to them; instead there is only a quick resolution where Cinderella is shown as the Prince's happy bride, her stepfamily out of the picture.

In this film, where she is voiced by Susanne Blakeslee,[2] Lady Tremaine only appeared in the An Uncommon Romance segment, where she plans to have both Drizella and Anastasia find wealthy suitors for Cinderella's incoming ball.

Furious at Anastasia's sudden conscience and Cinderella's interference, Lady Tremaine emerges from her hiding place and begins turning some of the palace guards into farm animals.

At first, Lady Tremaine did not seem to bear any particular animosity towards Ella, but her jealousy and spite was incited after overhearing a private conversation in which it was confirmed that her second husband loved his biological daughter more than he did her, and that he still cherished the memory of his first wife.

She then blackmails the Grand Duke into ensuring that she will become a countess and that her daughters will get wealthy husbands, in return for keeping the secret so that Kit can be married to a princess from another kingdom, as his father had originally intended.

Aside from the films, Lady Tremaine has also made various appearances on the animated series House of Mouse; she frequently shares a table with another famous wicked stepmother, the Evil Queen from Snow White and the Seven Dwarfs.

When Aqua jumps in and rescues Cinderella, the Cursed Coach accidentally drops a fire bomb in front of Lady Tremaine and her daughters, which explodes and (as revealed in Kingdom Hearts III) turns them into Heartless.

She also appears in Kingdom Hearts Union χ, fulfilling the same role as in the 1950 film and Birth by Sleep, as an image of the events that would happen in the future.

Lady Tremaine is the antagonist in the Disney Cruise Line Broadway-style stage musical Twice Charmed: An Original Twist on the Cinderella Story.

In the end credits of the animated short film, The Simpsons: May the 12th Be With You, Lady Tremaine appears along with the Evil Queen and Mother Gothel protesting against Disney being unfair to stepmothers.

A second version of Lady Tremaine appears as the main antagonist of the seventh and final season, portrayed by Gabrielle Anwar, with the character amalgamated with Rapunzel.