The conference's current lineup consists of five schools in northwestern Iowa.
Since Iowa is the only state in the nation that maintains separate governing bodies for boys' and girls' athletics, the classifications are different for each gender.
Sheldon officials cited travel distances as the reason for making the jump, as five schools from the Siouxland Conference are closer to Sheldon than the second-closest Lakes Conference school.
[7] Emmetsburg officials cited school size comparisons and a lack of ability to compete successfully in the Lakes Conference as the two main reasons for the switch.
On February 15, 2021, Western Christian announced it would be leaving the conference following the 2021-2022 school year.
The school did not specify which conference it would be joining, it did mention that it was interesting in competing more locally.
The school has attempted to join the Siouxland Conference, and was denied membership.
On December 17 2024 The Siouxland Conference extendend invitations to Cherokee, Estherville–Lincoln Central, Spencer and Storm Lake to join starting with the 2026-27 academic year.