Kitzingen is a Landkreis (district) in Bavaria, Germany.

It is bounded by (from the north and clockwise) the districts of Schweinfurt, Bamberg, Neustadt (Aisch)-Bad Windsheim and Würzburg.

The district in its present form was established in the administrative reform of 1973.

The former district of Gerolzhofen was dissolved, and half of its territory was merged with the Kitzingen district (which had been much smaller before).

The river Main runs through the district from north to south.