[1] Each week, hosts Rogers and Yang discuss popular culture, current events, and their personal lives.

"[2][3] Toward the end of every episode, there is a segment called I Don't Think So, Honey,[4] during which the hosts and any guests are each given sixty seconds to rant about an element of culture they find frustrating.

[5] Las Culturistas host occasional I Don't Think So, Honey Live shows featuring up to fifty local comedians performing their own versions of the segment.

[6] Every June since 2022, Yang and Rogers host an annual live parody award show in New York City called the Las Culturistas Culture Awards, which has featured live musical performances by Yang, Rogers, and others; guest appearances by fellow actors, comedians, podcasters, and musicians; and video acceptance speeches from minor and major celebrities including Taylor Swift, Andy Cohen, Ariana Grande, Alan Cumming, Vanderpump Rules' Ariana Madix, Tony Award-winning star of Kimberly Akimbo Bonnie Milligan, Cate Blanchett, and more.

[14] In March 2020, Las Culturistas returned with new episodes on Will Ferrell's Big Money Players podcast network with iHeart Radio.