It has been serialized in Kodansha's seinen manga magazine Morning since December 2022, with its chapters collected in four tankōbon volumes as of May 2024.

Originally written by Shogo Imamura based on his Ikusagami novel and illustrated by Katsumi Tatsuzawa [ja], Last Samurai Standing started in Kodansha's seinen manga magazine Morning on December 8, 2022.

[12] Cast is as follows: Junichi Okada as Shujiro Saga; Riho Yoshioka as Shujiro's wife, Shino; Yumia Fujisaki [ja] as Futaba Katsuki; Kaya Kiyohara as Iroha Kinugasa; Taichi Saotome as Shikura Adashino; Yuya Endo as Sansuke Gion; Masahiro Higashide as Kyojin Tsuge; Shota Sometani as Kocha Kamuy; Hiroshi Tamaki as Ukyo Kikuomi; Takayuki Yamada as Jinbe Ando; Kairi Jo as Shinjiro Sayama; Wataru Ichinose [ja] as Raizo Tachibana; Hideaki Itō as Bukotsu Kanjiya; Yasushi Fuchikami [ja] as Sakura; and Enju, played by Kazunari Ninomiya.

[13] Aki Nakano of Kodansha Comic Plus finds the Last Samurai Standing manga adaptation of Shōgo Imamura's novel a gruesome, vividly rendered death game set in the chaotic early Meiji period, with a great sense of pacing and mesmerizing characters.

[14] Zen Narita, in a review for Da Vinci News, praised Katsumi Tatsuzawa's manga art, describing them as "moment-to-moment", facial expressions and physical movements of the characters in battle as "dynamic and enjoyable.