Muscardin was awarded a graduate degree in modern history from the University of Rome.
[1] She made her debut in the cinema industry as assistant director for the feature film Una vita scellerata (A Violent Life) by Giacomo Battiato.
After directing the short Il cuore – Le coeur (The Heart) in 1998, in 2001 she directed her first feature film Giorni (Days), a romantic film about a man dying of AIDS, featuring Thomas Trabacchi and Riccardo Salerno.
In 2007 she directed the feature film Billo: Il grand Dakhaar, a comedy about immigration about a Senegalese fashion designer, played by real-life designer Thierno Thiam, torn between women in Rome and back home.
She has also directed a making-of documentary about Roberto Rossellini's film Rome, Open City.