[4] On 28 June 1894 at St. John's Church of England, Camberwell, she married Norman Thomas Mortimer Wilsmore, a fellow science graduate, who was later Professor of Chemistry at the University of Western Australia.
The occasion was a double wedding, as Little's sister, Grace Lillias Little married Herbert Foley Rodda.
[5] A paper she wrote, entitled "Barriers to migration, and their effects as shown in the Australian region", was published in The Victorian Naturalist in 1894 by the Victorian Naturalists' Club to which she had been elected member in 1893.
[4] When her only child, a son born in Scotland, had begun school, Little enrolled at University College London where she studied zoology and described seven new species sea anemones,[4] including Epiphellia browni,[6] Epiphellia capitata[7] and Peachia hilli.
[10] Upon their deaths, any residue was to be given to the University of Melbourne for them to set up the Norman Thomas Mortimer Wilsmore Research Fund,[11] in memory of her husband, who had predeceased her in 1940.