Lesbos Prefecture (Greek: Νομός Λέσβου) was one of the prefectures of Greece.
It comprised three main islands: Lesbos itself, Lemnos, and the smaller island of Agios Efstratios.
Its capital was the town of Mytilene, on Lesbos.
In 2011 the prefecture was abolished and the territory was divided between the regional units of Lesbos and Lemnos.
Note: Provinces no longer hold any legal status in Greece.