For the program tapings, the show's band of three musicians, which included Laugh-In's musical coordinator, Russ Freeman, all wore the yellow rain jackets and hats which had been worn as costumes by various characters as joke links on Rowan & Martin's Laugh-In.
One particularly notable joke from the program asked the question, "What's the difference between a sigh, a car, and a jackass?"
The eventual Grand Prize-winning entry was a joke read by actress Jill St. John: "What do you get when you cross an elephant with a jar of peanut butter?
Like Match Game, Letters to Laugh-In faced the popular Dark Shadows on ABC and reruns of Gomer Pyle, U.S.M.C.
The episode, taped on September 6, 1969, featured a celebrity panel of Jo Anne Worley, Dan Rowan, Angie Dickinson, and Jack E.