Lime Hollow, through a renewable use agreement, began by utilizing 100 acres (0.40 km2) of land and two buildings belonging to the Tunison Laboratory of Aquatic Science of the US Geological Survey.

Lime Hollow purchased an additional 190 acres (0.77 km2) adjoining the Tunison property in 1998, partly funded with a grant from the New York State Clean Air/Clean Water Bond Act.

The peat bog is home to a wide swathe of Labrador tea (Ledum groenlandicum), an acid-loving plant whose growth season is in the spring.

[5] In addition to the summer programs, local schools frequently send field trip groups to the center throughout the year.

[7] Lime Hollow has developed nearly 10 miles (16 km) of walking trails, several wildlife viewing stations, and over 375 acres (1.52 km2) of land.