The Leader of the Opposition in the Maharashtra Legislative Assembly is an elected member of the Maharashtra Legislative Assembly who leads the official opposition in the lower house of the Maharashtra Legislature.

The leader of the opposition is the legislature speaker of the party with the most seats after the government party.

The post is vacant since 26 November 2024 since no opposition party has even 10% of the total seats of the house.

This is commonly the leader of the largest non-government party, and is recognized as such by the speaker.

The following is the list of leaders of the opposition in the assembly.