This is a list of fictional non-binary characters (i.e. genderqueer) identified as such in anime, animation, print media, feature films, live-action television, theatre, video games, webcomics, and other mediums.
Queer identifications listed include characters of non-binary gender, agender, bigender, genderfluid, genderqueer, as well as characters of any third gender.
For more information about fictional characters in other identifications of the LGBTQ community, see the lists of lesbian, bisexual, gay, transgender, aromantic, asexual, intersex, and pansexual characters.
The names are organized alphabetically by surname (i.e. last name), or by single name if the character does not have a surname.
In the 2022 book Marcy's Journal: A Guide to Amphibia, Marcy uses gender-neutral pronouns for them.