In 2014, a $329,851 grant from the Growing Greener program was issued to the Union County Conservation District for agricultural best management practices in its watershed.

Little Buffalo Creek begins at the base of a mountain in White Deer Township.

The creek continues flowing south for some distance before meandering east for several tenths of a mile.

[2] Little Buffalo Creek is impacted by nutrients, sediment, E. coli, and thermal radiation.

[1] There are also significant flood-prone areas along the creek, which, during a flood, could make access to emergency services in western Kelly Township difficult.

[2] The mouth of the creek is in the United States Geological Survey quadrangle of Lewisburg.

[3] Little Buffalo Creek was entered into the Geographic Names Information System on August 2, 1979.

[8] A bridge built over the creek in 1850 was used as a route for transporting grain to Campbell's Mill.

A bridge carrying State Route 1003 was built over the creek 1 mile (1.6 km) north of Kelly Cross Roads in 1934.

A two-span bridge was built across the creek 2 miles (3.2 km) south of Kelly Cross Roads in 1949, was repaired in 2002, and is 78.9 feet (24.0 m) long.

In 1950, a bridge carrying State Route 1004 was constructed over the creek in Kelly Cross Roads and is 58.1 feet (17.7 m) long.

In 1951, a bridge carrying State Route 1006 was built over Little Buffalo Creek in 1.5 miles (2.4 km) northeast of Kelly Cross Roads and is 54.1 feet (16.5 m) long.

[10] A bridge with a length of 78.1 feet (23.8 m) was built across Little Buffalo Creek in 1985 and carries State Route 1003 1 mile (1.6 km) west of Kelly Point.

A bridge with a length of 61.0 feet (18.6 m) was built over the creek in 1991 and carries T-391 0.9 miles (1.4 km) east of Kelly Point.

[3] In 2014, the a $329,851 grant from the Growing Greener program was issued to the Union County Conservation District for agricultural best management practices in the creek's watershed.

The aim of the project was to restore American eels and freshwater mussels to the ecosystem of the Susquehanna River.

[15] In a 2008 study, Little Buffalo Creek was found to be less biologically impaired than expected given the amount of agricultural activity and livestock in its watershed.

Additionally, some wetlands have been created along the creek by landowners to serve as waterfowl habitats and the Conservation Reserve Enhancement Program of the United States Department of Agriculture has installed riparian buffers on the creek in some formerly agricultural land.