Little Fishing Creek begins on Huckleberry Mountain in Davidson Township, Sullivan County.

It flows south and exits Davidson Township and Sullivan County within a few tenths of a mile.

After several miles, it starts flowing parallel to Pennsylvania Route 42 receives the tributary Branch Run.

The creek then passes by the western edge of Millville and continues flowing south roughly parallel to Pennsylvania Route 42.

Several miles downstream, it passes Eyers Grove, where it receives the tributary Spruce Run and leaves behind Greenwood Township.

After some distance, the creek passes Mordansville and reaches the southern border of Mount Pleasant Township several miles later.

[7] All but one of the tributaries of the Little Fishing Creek upstream of the United States Geological Survey stream gauge empty into it from the right.

[7] A total of 2.4 miles (3.9 km) of Little Fishing Creek are considered impaired due to pathogens of an unknown source.

[12] The average annual rate of precipitation in the watershed of Little Fishing Creek ranges from 35 to 45 inches (89 to 114 cm).

[13] The annual recharge levels of Little Fishing Creek at Eyers Grove ranged from 6 to 23 inches (15 to 58 cm) between 1941 and 1957.

[14] Between its headwaters and Talmar Road, the concentration of alkalinity in the waters of Little Fishing Creek is 11 milligrams per liter.

[17] All of the rock in the creek's watershed upstream of the United States Geological Survey's stream gauge is shale or sandstone.

Rock outcroppings of the Chemung Formation are visible in Hemlock Township, approximately 1 mile (1.6 km) upstream of Fishing Creek.

The drainage basin area of the creek upstream of the stream gauge at Eyers Grove is 56.5 square miles.

[8] The watershed is part of the Lower North Branch Susquehanna River drainage basin.

[19] Major communities in the watershed of the creek include Millville, Iola, Eyers Grove, and Mordansville.

[13] Between its headwaters and Talmar Road, 6 percent of the creek's length is on open public land.

[2] This variant name appears in a 1982 map of Columbia County published by the Pennsylvania Department of Transportation.

[23] The headwaters of Little Fishing Creek were uninhabited considerably after the valleys of Greenwood and Jerseytown were inhabited.

[28] Little Fishing Creek serves as a water supply source for nearby communities such as Millville.

The Susquehanna, Bloomsburg, and Berwick Railroad historically crossed the watershed and followed the creek between its mouth and Millville.

[13] The drainage basin of Little Fishing Creek is designated as Exceptional Value waters and a Migratory Fishery upstream of the tributary Lick Run in the community of Sereno.

[29] The creek is considered by the Pennsylvania Fish and Boat Commission to be Class A Wild Trout Waters from its headwaters to Talmar Road: a distance of 4.3 miles (6.9 km).

[15] Ruffed grouse occur in large numbers on Little Fishing Creek north of Iola.

[9] It is possible to canoe on 11.4 miles (18.3 km) of Little Fishing Creek during snowmelt or within two days of heavy rain.