It previously served the Pennsylvania Railroad (PRR) and later SEPTA Regional Rail's R3 West Chester Line.

In 1997, this portion of the line was reopened by the West Chester Railroad heritage railway for weekend excursions; the company restored the Locksley station building.

Pennsylvania Railroad established Locksley station on May 24, 1890, based on a petition from the residents of the locality.

The name, according to a 1901 newspaper article, did not originate from the area, but was chosen by the superintendent of that division of the railroad from a volume of poems by Alfred, Lord Tennyson.

A PRR historic manual block position-light signal stands near the station site.