[5] LocoCycle is an action-adventure beat-'em-up game where players control the main character, a sentient combat motorcycle named I.R.I.S, to fight against a military weapons company seeking to destroy her.

Also, I.R.I.S will occasionally break down, and the player will have to perform a series of quick time events as Pablo in order to repair her.

At the same time, Pablo's pant leg gets stuck in I.R.I.S' chassis, literally dragging him along as the bike escapes the facility.

Meanwhile, S.P.I.K.E also gains a human companion of his own to match I.R.I.S in combat by borrowing a vacationing woman named Ella Mae.

Arriving at the Freedom Rally, an overjoyed I.R.I.S discovers a sinister plot by Holt Ryebach, one of the guests at the unveiling party, who plans to use mass-produced I.R.I.S and S.P.I.K.E models, as well as a legion of missiles, to attack a biker club in Janesville, Wisconsin with a bomb strike, as revenge for refusing to join them.

S.P.I.K.E also returns Ella Mae to her motor home, where she is greeted by her grandchildren, and decides to travel the country for sightseeing.