[1] London Street Railway Company (LSR), a privately operated transit service, brought public transit to the city with the start of horse-drawn streetcar operations May 24, 1875, on Dundas Street.

[3] Privately owned from 1875 to 1920, the Ontario Railway and Municipal Board took over control of LSR in 1920.

In 1951, the city assumed control over all routes and formed the London Transportation Commission to operate them.

The streetcar system was fully converted to buses in late November 1940 (originally planned for the end of 1940, but hastened by a blizzard that damaged trolley wires).

[3] The system has evolved to feature community bus routes, para-transit services and accessible low floor buses with the express lines as the backbone.

An economic slump echoed in a parallel drop in ridership, made even worse by the traditional responses of service cuts and fare increases.

Innovations included an overhauling of fare structure, re-thinking routes, bringing buses into mall areas (which would later become true terminal areas), and making standard public transit buses increasingly wheelchair-accessible.

Post-secondary students attending the University of Western Ontario and Fanshawe College now receive a subsidised bus pass incorporated into their tuition a program which has become a great success.

[6][7] For safety of the drivers and to limit the spread of the coronavirus paying a fare was suspended.

[8] The London Transit Commission announced that in August front door boarding will continue with new driver barriers installed and a yellow line to help with social distancing requiring passengers to stay behind the yellow line.

It initially operated between downtown and Masonville Mall via Richmond, with limited stops.

In addition, Route 91 was added to run on Oxford Street between Wonderland Road and Fanshawe College.

In 2015, Route 92 was introduced, running from Masonville Mall to Victoria Hospital, largely via Adelaide Street.

In 2021 London Transit introduced Route 95 a new peak period semi express bus that will operate between White Oaks Mall and Fanshawe College via Bradley Ave and Highbury Ave as part of the 2021 service changes.

Construction is planned to begin in 2021 and it is estimated that it will be fully completed by 2028[11] In October 2021 London Transit has partnered with MagusCards to help people for those with autism or other cognitive special needs.

The strike affected all public routes; however, specialized transit services for the disabled continued to operate.

A community-based approach was taken by the USC including a flag-a-ride program and a shuttle service for groceries.

David Empey, president of the UWO staff association, was against this volunteer service, calling it "scab labour".

"The strike is really inconveniencing people who are paid even less than the drivers," said a third-year Western student.

[14] On December 7, 2009, ATU turned down the LTC's "Final Offer" of 9.3% wage hike over three years.

Route 10 buses serve Masonville Mall Monday to Friday during afternoons and early evening only.

Route 10 buses serve Masonville Mall Saturday and Sunday during daytime service only.

Route 30 operates during peak periods and late evening service on Monday and Fridays.

The following regularly scheduled limited-service routes operate to provide special access to seniors and individuals with impaired mobility to major shopping destinations.