The Longleaf Trace is a 44-mile (71 km)[1] paved pedestrian, equestrian, rollerblade, and bicycle trail between Hattiesburg and Prentiss, Mississippi, United States.

Constructed in 2000, the trail follows a portion of the abandoned Mississippi Central Railroad line.

In Hattiesburg there are rest stops and parking lots accessing the trail at the University of Southern Mississippi, at West 4th Street, and at Jackson Road.

Wildlife such as wild rabbits, squirrels, raccoons, songbirds, and deer can be seen along the trail.

The trail also supports a variety of flora, including flowering dogwood and magnolia trees and fragrant honeysuckle and wisteria.