In addition to her father, Lore Uphoff-Schill received instruction in painting from Lothar von Kunowski at the Kunstgewerbeschule Düsseldorf.
In 1913, she and her husband Fritz Uphoff began to devote themselves to figurative representation in the studio they rented in Düsseldorf.
She also took her easel to the surrounding villages and created unique paintings in oil and a special spray technique depicting the moorland landscape around Worpswede.
During the economically difficult 1920s, Lore Uphoff was involved in the work of the "Werkgemeinschaft Worpswede für Buchkunst, gestochene Buch- und Mappenwerke" (Worpswede Association for Book Art, Engraved Book and Portfolio Works) founded by Fritz and his brother Carl Emil Uphoff.
In 1928, the Provinzialmuseum Hannover acquired 10 paintings by Worpswede artists, including Emmy Meyer, Martha Vogeler, Lore Uphoff-Schill and Carl Emil Uphoff.