Sabbatini was born in Bologna and studied with Prospero Fontana, who was his teacher and collaborator, and was a friend of Orazio Samacchini.
By 1565 he was working with the studio of Giorgio Vasari in Florence, where was elected member of the Academy.
[3] Between 1566 and 1573 he was in Bologna,[1] where he decorated the walls of several churches, including Santa Maria delle Grazie, Chiesa della Morte, San Martino Maggiore, and San Giacomo Maggiore.
In 1573 he moved to Rome to work under Vasari in the Cappella Paolina (with Federico Zuccari) and Sala Regia of the Vatican,[1] where he adopted many of the stylistic traits of Raphael's school and produced perhaps his most famous painting, The Triumph of Faith over Infidelity.
His students included the engraver Giulio di Antonio Bonasone and the painter of Flemish origin, Denis Calvaert.