Though it thrived in its early years, the Civil War took a great toll and by 1870 there were only six students enrolled, and as few as one professor at times, requiring classes to be taught by the faculty of Centre College.

In 1893, a seminary opened in Louisville, operating out of Sunday School rooms in Second Presbyterian Church at Second and Broadway, with 31 students and six professors initially, and an endowment of $104,000.

Because of the merger, it was the only institution supported simultaneously by the northern and southern branches of the modern Presbyterian Church (USA).

[5] Dean K. Thompson was named eighth president of Louisville Presbyterian Theological Seminary on April 22, 2004 and began his term in June 2004.

This campaign aimed to ensure the long-term financial health of the seminary and featured the creation of the Covenant Scholarship program, which was established in 2015.

[8] Alton B. Pollard, III is an American theologian and educator, best known for his service as the 10th president of Louisville Presbyterian Theological Seminary.

Pomerville began his tenure on July 1, 2023, and was formally confirmed by the 226th General Assembly of the Presbyterian Church (U.S.A.) (PCUSA) in 2024.