Lower Slaughter is a village in the Cotswold district of Gloucestershire, England, 4 miles (6.4 km) south west of Stow-on-the-Wold.

At the west end of the village there is a 19th-century water mill with an undershot waterwheel and a chimney for additional steam power.

While the mill is built of red brick most of the 16th and 17th century homes in the village use Cotswold limestone and are adorned with mullioned windows and often with other embellishments such as projecting gables.

Lower Slaughter Manor, a Grade-II listed 17th-century house, was granted to Sir George Whitmore in 1611 and remained in his family until 1964.

In 2013, the Parish Council opposed an icebox tricycle selling ice creams seven days a week, six months of the year, arguing that the trading times were excessive, increased footfall would prevent the grass from growing and children could climb on the trike and fall into the nearby river.