The Eye rises in a spring in the Cotswold Hills at a height of around 160m in the northern end of the parish of Upper Slaughter.

It flows south in its own steep-sided valley through the grounds of Eyford Park, a house built in the early 20th century, with noted formal gardens.

[2] Crossing the B4068, the river joins the footpath known as "Warden's Way", that runs from Bourton-on-the-Water to Winchcombe and after a kilometre passes St Peter's, parish church of the small village of Upper Slaughter, as it encircles the village to the north and east.

Turning south east, it descends into Lower Slaughter after another kilometre passing first under a former water mill.

There was a mill listed on the site in the Domesday Book, and it continued to produce flour for the village until the death of the owner in 1958.