Chambers made 792 appearances in the English Football League, placing him in the top ten list of all time.

During the 2007–08 season, Chambers was played regularly at right-back as part of a Forest defence that kept a record 24 clean sheets, eventually earning them promotion to the Championship.

This was not enough to keep him in the team however, and the return of Kelvin Wilson from injury, combined with the presence of Chris Gunter at right-back, saw Chambers end the season with a limited number of appearances.

[16] Chambers started the 2010–11 season mainly as a substitute, however due to injuries and the poor form of Kelvin Wilson, he was given an opportunity at centre-back.

[17] He impressed with consistent performances, keeping Wilson out of the first team and becoming a first choice, alongside Wes Morgan, in the centre of Forest's defence.

[22] However, at the end of a difficult season for Forest, he turned down the offer of a new contract, and at midnight on 30 June 2012 Chambers became a free agent.

With his contract at Forest set to expire, Chambers was approached by several other Championship sides, before undertaking a medical at Portman Road on 3 July 2012.

[28] Playing as a central defender, Chambers was part of an Ipswich defence that kept 12 clean sheets in the last 22 games of the 2012–13 season.

Chambers kept his starting place at right back for the entire 2013–14 season, making 48 appearances in all competitions, scoring 3 goals.

He made 48 appearances across all competitions, scoring 3 goals, including a 90th-minute winner over Queens Park Rangers at Portman Road on Boxing Day 2015.

He made a total of 39 appearances in all competitions during the season, including scoring in a 1–1 draw against local rivals Norwich City at Carrow Road on 18 February 2018.

[36] He was forced to miss out on the final five games of the season due to suffering a cracked rib, following a collision with Ipswich goalkeeper Bartosz Białkowski during a 1–0 loss to Brentford at Griffin Park on 7 April 2018.

[39] On 11 August 2018, in a match against Rotherham United at Portman Road, Chambers became Ipswich Town's highest appearance maker this century, having played 281 games for the club.

[42] Hurst was sacked and replaced by Paul Lambert, who was unable to reverse Ipswich's poor form, and the club was relegated to EFL League One.

[43] He scored his first goal of the 2019–20 season on 17 August, netting a 94th-minute equaliser in a 2–2 draw with Peterborough United at the London Road Stadium.

[47] On 20 June 2021, Chambers joined fellow East Anglian club Colchester United on a free transfer, reuniting him with former teammates Cole Skuse, Tommy Smith and Dean Gerken.

[50] On 12 May 2023, Colchester confirmed that Chambers would be released upon the expiration of his contract, having made 96 appearances for the club in all competitions during his two year spell.