The Madonna del Sacco is a fresco painting created in 1525 by the Italian Renaissance artist Andrea del Sarto in the Chiostro Grande (also called Chiostro dei Morti) adjacent to the Basilica of Santissima Annunziata in Florence.

Andrea del Sarto (1486–1530) had frescoed seven lunettes during 1514–1516 in the small cloister in front of the church facade, including the well-known Nativity of the Virgin.

The painting was depicted in copies and engravings by multiple artists including Raphael Morghen and Michelle Corneille the Elder.

The work depicts a forward-facing Madonna, seated on a ledge, with the Christ child moving across her lap.

To the right of the triangular composition, St Joseph leans on a white sack, reading from an open book.