The altarpiece was commissioned after 1344 by Margrave John Henry of Luxembourg for the romanesque church of Assumption of Our Lady, In the neighborhood of royal Veveří castle in Brno, Moravia, Czech Republic.

Albert Kutal a Czech scholar called it "a truly rare and extraordinary work".

The Virgin Mary wears double crown on uncovered loose hair, without a veil.

Some Byzantine-Italian traces notwithstanding, the painting shows a further step in the assimilation of Italian influences, which are adapted in an individually and stylistically balanced manner.

Pigment analysis of Master of Vyšší Brod's masterpiece reveals the usual pigments of the high gothic period such as ferric oxide mixed with orpiment in the red drapery on top of the painting, natural ultramarine mixed with lead white in the blue robe of Madonna (inside).