Malchiostro Annunciation is an oil on panel painting by the Italian Renaissance artist Titian, completed c. 1520.

The Malchiostro Chapel, located to the right of the high altar of the Cathedral of Treviso, near the sacristy, was commissioned by Broccardo Malchiostro, secretary of the bishop and humanist Bernardo de' Rossi.

Designed by Tullio of Antonio Lombardo, it was completed in 1519 and frescoed by Il Pordenone until 1520.

The decoration was completed in around 1523, and this Annunciation was executed by Titian around that period, perhaps with the help of Paris Bordone.

The painting depicts the Annunciation in a church with a chessboard pavement.