In some sources she is confused with her cousin, the mayor's daughter Mary Agnes Gilroy Mulqueen (1865–1938).

[3] She had roles in Only a Farmer's Daughter (1885), The Fakir (1890),[4] Romany Rye (1891),[5] Tuxedo (1891–1892),[6] Babes in the Woods (1893),[7] A Milk White Flag (1894),[8] Davy Jones (1894),[1] The China Dog (1895),[9] Little Miss Busybody (1895),[10] The Strange Adventures of Miss Brown (1896),[11] The Merry-Go-Round (1896),[12] Miss Manhattan (1897), Trilby (1898),[13] Mam'selle 'Awkins (1900), Star and Garter (1900),[14] El Capitan (1901), The Giddy Throng (1901),[15] The Girl from Paris (1898, 1902),[16] Lady Bountiful (1902),[17] and George W. Lederer's Mid-Summer Night Fancies (1903).

[18][19] The Boston Globe called Gilroy "one of the brightest, most vivacious, and altogether most charming soubrettes on the American stage.

[20] In 1901, her face, name, and words were used in print advertisements for Dr. Greene's Nervura, a "blood and nerve remedy" marketed to women.

[2][26] The "Mamie Gilroy" cocktail was named for the actress; it consists of whiskey, ginger ale and lime.