Mostly a gravel road, it connects the communities of Clanwilliam, Polonia, Eden, Plumas, and Langruth, as well as providing a connection between Riding Mountain National Park and Lake Manitoba.

PR 265 begins in the Rural Municipality of Minto-Odanah at an intersection with PR 262 just north of Clanwilliam, heading due east through farmland to enter the Rural Municipality of Rosedale and traverse a small river valley, where it passes through Polonia.

The highway continues east across several small streams to enter the town of Eden, where it joins a short concurrency with PTH 5 (Parks Route) as it passes along the western side of town.

PR 265 travels along the northern side of the town of Plumas along Queens Road and Clear Avenue, where it crosses a railroad, before joining a concurrency with PR 260 for few kilometres as their leaving town.

PR 265 now traverses the swampy marshlands surrounding Jackfish Lake, where it crosses the Whitemud River, before entering the hamlet of Langruth, where it comes to and end at a junction with PTH 50 (Kinosota Road).