[8] Ledger had noted Mamani's knowledge in a letter where he recorded asking him for his opinion on finding good quality cinchona trees in the area they were staying.
[1] Mamani waited through four years of unsuitable weather (frosts destroyed the seeds from the high-quinine plants), and gave offerings to mountain spirits, in order to obtain a sample of seed from the high-quinine cinchona in 1865.
[7][9][10] The seeds that Mamani provided were sent to Ledger's brother, George, who then sold them to the Dutch government, who then cultivated plants in Java.
[3][12] The plant from which Mamani collected seed was later named Cinchona ledgeriana (syn C. calisaya) after Charles Ledger.
[13] One researcher has suggested that 'Incra Mamani', as spelled by Charles Ledger in his letters, may be an Anglicization of 'Icamanahí'.