Matamoros was born in Lepe in Huelva as the son of a lieutenant colonel in the Spanish Artillery and he was brought up at Málaga in Andulusia.

[1] He became enthusiastic about Protestantism following figures such as Francisco de Paula Ruet and Antonio Vallespinosa and he is said to have been converted in Gibraltar.

[1] In a strict Catholic country he was sentenced in Granada to eight years and banned from being a teacher.

Matamoros left behind an extensive collection of Carte de Visite for Protestants he had met in Lausanne, Gibraltar and elsewhere.

William Harris Rule, a Methodist minister who had tried unsuccessfully to introduce Protestantism into Spain twenty years before Matamoros.