Marbled salamanders are found in the eastern United States, from southern New England to northern Florida, and west to Illinois and Texas.

[6] The first months that Marbled Salamanders spend living out of the water are the most important in determining how many will survive until the next breeding season.

[7] Desiccation, heat stress, soil moisture, temperature, and pH are all important factors in determining if a Marbled Salamander will survive.

[8] Conservationists recommend leaving a buffer zone of forest around wetlands to increase survivorship of Marbled Salamanders.

Marbled salamanders will migrate to seasonal pond basins in the late summer and early fall where they will court and deposit eggs.

[8] Because Marbled Salamanders have relatively long life spans, their chances of extinction due to catastrophic failure are low.

[16] This dispersal also means that it is important to view these populations as a larger metapopulation, rather than focusing simply on a single wetland area.

[17] Adults feed on terrestrial invertebrates, such as worms, insects, centipedes, other arthropods, and mollusks (snails, slugs).

[20] When A. opacum is under attack by a predator, they often exhibit tail lashing, head-butting, body coiling, or potentially becoming immobile.

These defensive moves are thought to draw attention to the tail, which has granular glands that produce noxious secretions to protect themselves.

A problem with the granular glands Marbled Salamanders possess is that secretions are reduced after multiple attacks, making them more vulnerable.

[21] Marbled salamanders have been found to exhibit prominent biofluorescence under ultraviolet excitation along the bones in their digits and the cloacal region of both males and females.