In 1885, she became one of the first women to go up to Cambridge University, where she read Modern and Medieval Languages at Newnham, gaining the equivalent of a first class honours degree in 1888.
Tuke began her academic career at Newnham College and taught French there, as a staff lecturer in modern languages, from 1890 to 1905.
During her time there she oversaw its move from Baker Street, London, to a site at Regent's Park, after which the number of students doubled.
[4] While overseeing the expansion of the academic provisions at the College, she also served as the representative of the Faculty of Arts on the University of London Senate from 1911 until her retirement.
[2] In September 1937 Dame Margaret presented the Library of Royal Holloway, University of London with a collection of Italian Renaissance letters dating from 1526 to 1697.
Descriptions of their friendship and letters exchanged between the two are included in 'A Supressed Cry- The Life of a Victorian Daughter', the biography of Seebohm authored by her great-grand-niece, Victoria Glendinning.